Microtubule arrays during the first cell cycle in the green urchin Strongylocentrotus droebachiensis.

The green urchin zygote has a diameter of about 160 µm. These large cells offer excellent spatial and temporal resolution for cytokinesis studies. This gallery of single focal plane confocal micrographs shows that during anaphase microtubules contact the cell poles before they contact the equator and that furrowing commonly initiates a little before microtubule tips touch the equatorial cortex. Comparison with the companion gallery for purple urchin zygotes, whose diameter is half that of the green urchin zygote shows that the smaller cells initiate cleavage earlier with respect to nuclear cycle phase and that when furrowing begins in purples microtubules are normally touching the equator. The final panel in this gallery displays a green urchin zygote and a purple urchin zygote, at the same magnification, both at incipient furrowing. All images were made with a BioRad Radiance 2000 laser scanning confocal microscope on a Nikon E800 microscope using a 60X 1.4 oil Plan Apo objective. Preparation protocol is described in Foe and von Dassow, 2008.

Please note: images on this page belong to Victoria Foe. You are welcome to use them in your own presentations/lectures with appropriate credit.

The thumbnail images (150X150 pixels) below link to high-resolution images that will open in a new window – (1024X1024 pixels; approx. 275KB).

1interphase

1 interphase

2

2 incipient prophase

3prophase

3 prophase

4metaphase

4 metaphase

5anaphase

5 anaphase

6anaphase

6 anaphase

 
7anaphase

7 anaphase

8telophase

8 telophase

9telophase

9 telophase

10telophase.jpg

10 telophase

11interphase

11 interphase

12interphase

12 interphase

 
13interphase

13 interphase

14interphase

14 interphase

green and purple

green and purple

 
 

simulations   •   live-label movies   •   time-lapse movies   •   C. elegans   •   Dendraster   •   confocal 3D movies   •   still images   •   green urchin MTs   •   purple urchin MTs   • invert embryology   • plankton   •